We estimated the uptake lengths (S_w) of NO₃-N and PO₄-P using the nutrient enrichment method to assess their transport dynamics in Panther Creek (forested stream) and Ledbetter Creek (agricultural stream). The agricultural stream had higher ambient NO₃-N than the forested stream, while PO₄-P was higher in the forested stream than agricultural stream. Although we expected shorter S_w estimates in the forested stream based on the ambient nutrient concentrations and higher metabolism, the agricultural stream (Ledbetter) had shorter S_w (372 m for N and 327 and 242 m for P) than estimates from Panther Creek (753 and 870 m for N; 827 m for P). Nutrient uptake patterns in these streams may reflect the combined effects of biological activities and hydrologic characteristics. Ledbetter Creek contained several large pools and backwater areas that allowed longer residence and contact time for amended nutrients than Panther Creek. It was also assumed that Ledbetter had a potential for higher abiotic phosphorus adsorption and microbial denitrification due to the presence of large pools and finer substrate composition along the study reach.